MacConkey agar with sorbitol is a selective and differential culture medium used to aid in the isolation and identification of the gram-negative bacterium called Escherichia coli 0157:H7.
This medium contains a pH indicator, crystal violet, bile salts, lactose, and sorbitol. Lactose is a fermentable sugar, while sorbitol is a non-fermentable sugar. The presence of lactose in the medium allows for the selective growth of gram-negative bacteria, while crystal violet and bile salts inhibit the growth of gram-positive bacteria.
The addition of sorbitol makes it possible to differentiate between E. coli O157:H7 and other non-pathogenic types of E. coli, as the strain produces a distinct non-sorbitol-fermenting phenotype. This means that E. coli O157:H7 will not produce acid from sorbitol fermentation and will appear as colorless colonies, while other strains of E. coli will appear pink-red colonies.
MacConkey agar with sorbitol is commonly used in clinical laboratories to diagnose and confirm the presence of E. coli O157:H7 in stool samples of patients with diarrhea. Prompt identification of this pathogen is important as it can cause severe gastrointestinal illness and potentially fatal complications.
Ne Demek sitesindeki bilgiler kullanıcılar vasıtasıyla veya otomatik oluşturulmuştur. Buradaki bilgilerin doğru olduğu garanti edilmez. Düzeltilmesi gereken bilgi olduğunu düşünüyorsanız bizimle iletişime geçiniz. Her türlü görüş, destek ve önerileriniz için iletisim@nedemek.page